今天是:
师资队伍
当前位置: 首页 > 师资队伍 > 教学科研人员 > 副教授 > 正文

姓名:张伟锋

职称/职务:副教授

电话:18191276305

传真:

电子信箱:zweifeng@snnu.edu.cn

研究方向:脑胶质瘤的免疫逃逸机制;脑胶质瘤的免疫治疗

办公地点:格物楼3407

 

一、个人简介

2014年获得博士学位,并于2017-2020年在著名的神经免疫学专家A.M. Rostami教授实验室学习,该实验室在全美多发性硬化研究领域名列前茅。从事基因治疗以及神经系统疾病发病机制研究多年,在国际顶级期刊Sci. Trans. Med., Sci. Immunol., Cell. Mol. Immunol., Frontiers in Immunology, Molecular Therapy - Methods & Clinical Development等发表论文近20篇;申报专利8项,已授权7项;主持或参与国家自然科学基金、陕西省自然科学基金、中央高校基金等科研项目14项;担任Molecular Therapy-Nucleic AcidsInternational Journal of Biological SciencesMolecular Therapy - Methods & Clinical Development等杂志的审稿人。

 

二、主要研究领域及兴趣

脑胶质瘤的免疫逃逸机制;脑胶质瘤的免疫治疗

 

三、承担的科研项目

1. 国家自然科学基金面上项目,81771345,黄芩苷促进少突胶质前体细胞分化及髓鞘再生 的PPARγ/NFIL3信号转导机制,2018/01/01-2021/12/31,主要参与人;

2. 国家自然科学基金面上项目,81773265,基于双靶分子识别的Dox调控的增强型IL13 CAR-T在恶性脑胶质瘤动物模型中的实验治疗研究,2018/01/01-2021/12/31,主要参与人;

3. 国家自然科学基金青年项目,31601086,携带CRISPR/Cas和供体DNA的高容量腺病毒介导的体内细胞高效靶向修饰方法的探究,2017/01-2019/1218万元,主持;

4. 中央高校基金“自由探索项目”,GK201603113,新型多位点打靶腺病毒载体的构建及研究,2016/01-2017/12,主要参与人;

5. 国家自然科学基金面上项目,81471772,结合靶向基因组编辑技术构建用于单基因缺陷原位修复的新型高容量腺病毒载体,2015/01-2018/12,主要参与人;

6. 国家自然科学基金面上项目,31470058,二酰甘油激酶θ参与非酒精性脂肪肝的发生与缓解过程中的分子机制,2015/012016/12,主要参与人;

7. 陕西省自然科学基金,2015JQ8302,二酰甘油激酶DGKθ参与糖、脂代谢调控的分子机制,2015/012016/12,主要参与人;

8. 国家自然科学基金面上项目,81272543,骨髓间充质干细胞装载新型药物诱导性溶瘤腺病毒载体Ad5/11-Ind-D24靶向脑胶质母细胞瘤的实验治疗研究,2013/012016/12,主要参与人;

9. 中国博士后科学基金面上项目一等资助,2015M570809,用于多位点基因打靶的新型腺病毒载体的构建及应用研究,2015/05-2016/06,主持;

10. 中央高校基金“自由探索项目”,GK201503041P53Pten缺失小鼠原位肝癌模型的建立,2015/01-2016/12,主持;

11. 中央高校基本科研业务费创新团队项目,GK201301010TALEN技术在GUS突变的小鼠模型中的实验治疗研究,2013/01-2015/12,主要参与人;

12. 中央高校基本科研业务费重点项目,GK200901009,锌指酶技术在血友病动物模型中的基因治疗研究,2009/01-2012/12,主要参与人;

13. 陕西省自然科学基金,2007C201,双表达VEGF siRNA 的新型靶向Ad5/3-RGD腺病毒载体在脑胶质母细胞瘤基因治疗中的实验研究,2008/01-2010/12,主要参与

 

四、出版的著作

1. 《分子系统学》,2012,科学出版社,独著作(主编、副主编、参编)

 

五、代表性论文

1. Rasouli, J., Casella, G., Ishikawa, L. L. W., Thome, R., Boehm, A., Ertel, A., Melo-Silva, C. R., Mari, E. R., Porazzi, P., Zhang, W., Xiao, D., Sigal, L. J., Fortina, P., Zhang, G. X., Rostami, A.,Ciric, B. (2021) IFN-beta Acts on Monocytes to Ameliorate CNS Autoimmunity by Inhibiting Proinflammatory Cross-Talk Between Monocytes and Th Cells. Front Immunol, 12, 679498. IF=7.5

2. Xiao D#, Zhang W#, Wang Q, Li X, Zhang Y, Rasouli J, Casella G, Ciric B, Curtis M, Rostami A, Zhang G-X. CRISPR-mediated rapid generation of neural cell-specific knockout mice facilitates research in neurophysiology and pathology. Molecular Therapy - Methods & Clinical Development 2021, 20: 755-764. IF=6.7

3. Rasouli, J., Casella, G., Yoshimura, S., Zhang, W., Xiao, D., Garifallou, J., Gonzalez, M.V., Wiedeman, A., Kus, A., Mari, E.R., Fortina, P., Hakonarson, H., Long, S.A., Zhang, G.X., Ciric, B., Rostami, A. (2020) A distinct GM-CSF(+) T helper cell subset requires T-bet to adopt a TH1 phenotype and promote neuroinflammation. Sci Immunol, 5. IF=17.7

4. Li, X., Zhang, Y., Zhang, W., Xiao, D., Ciric, B., Rostami, A., Zhang, G.X. (2020) P7C3 attenuates CNS autoimmunity by inhibiting Th17 cell differentiation. Cell Mol Immunol, 10.1038/s41423-020-0497-5. IF=11.5

5. Casella, G., Rasouli, J., Thome, R., Descamps, H.C., Vattikonda, A., Ishikawa, L., Boehm, A., Hwang, D., Zhang, W., Xiao, D., Park, J., Zhang, G.-X., Alvarez, J.I., Rostami, A., Ciric, B. (2020) Interferon-γ/Interleukin-27 Axis Induces Programmed Death Ligand 1 Expression in Monocyte-Derived Dendritic Cells and Restores Immune Tolerance in Central Nervous System Autoimmunity. Front Immunol, 11, 2425. IF=7.5

6. Casella, G., Rasouli, J., Boehm, A., Zhang, W., Xiao, D., Ishikawa, L.L.W., Thome, R., Li, X., Hwang, D., Porazzi, P., Molugu, S., Tang, H.-Y., Zhang, G.-X., Ciric, B., Rostami, A. (2020) Oligodendrocyte-derived extracellular vesicles as antigen-specific therapy for autoimmune neuroinflammation in mice. Science Translational Medicine, 12, eaba0599. IF=17.9

7. Li, Y., Zhang, W., Zhao, J., Li, S., Shan, L., Zhu, J., Li, Y., Zhu, H., Mao, Q., Xia, H. (2018) Establishing a dual knock-out cell line by lentivirus based combined CRISPR/Cas9 and Loxp/Cre system. Cytotechnology, 70, 1595-1605. IF=2.0

8. Zhang, W., Xiao, D., Shan, L., Zhao, J., Mao, Q., Xia, H. (2017) Generation of apoptosis-resistant HEK293 cells with CRISPR/Cas mediated quadruple gene knockout for improved protein and virus production. Biotechnol Bioeng, 114, 2539-2549. IF=4.5

9. Xiao, D.#, Zhang, W.#, Li, Y., Liu, K., Zhao, J., Sun, X., Shan, L., Mao, Q., Xia, H. (2016) A novel luciferase knock-in reporter system for studying transcriptional regulation of the human Sox2 gene. J Biotechnol, 219, 110-116. (co-first author) IF=3.503

10. Chen, F., Zhang, W., Zhao, J., Yang, P., Ma, R., Xia, H. (2016) [Construction of Rev-erbbeta gene knockout HEK293 cell line with CRISPR/Cas9 system]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi, 32, 1446-1452.

11. Zhang, W., Wang, D., Liu, S., Zheng, X., Ji, H., Xia, H., Mao, Q. (2014) Multiple copies of a linear donor fragment released in situ from a vector improve the efficiency of zinc-finger nuclease-mediated genome editing. Gene Ther, 21, 282-288. IF=5.2

12. Zhang, W., Chen, H., Zheng, X., Wang, D., Ji, H., Xia, H., Mao, Q. (2014) Targeted genome correction by a single adenoviral vector simultaneously carrying an inducible zinc finger nuclease and a donor template. J Biotechnol, 188, 1-6. IF=3.503

13. Zhang, W., Zheng, X., Wang, Y., Mao, Q., Xia, H. (2012) Establishment of a cell line carrying single copy of an exogenous mutant reporter gene for assaying the biological activity of ZFNs. J Biotechnol, 162, 191-196. IF=3.503

14. Zhang, W., Guo, Y., Zhang, C., Ji, H., Meng, W., Wang, D., Li, X., Mao, Q., Xia, H. (2012) Rescue the failed half-ZFN by a sensitive mammalian cell-based luciferase reporter system. PLoS One, 7, e45169. IF=2.74

15. Li, X., Mao, Q., Wang, D., Zhang, W., Xia, H. (2012) A fiber chimeric CRAd vector Ad5/11-D24 double-armed with TRAIL and arresten for enhanced glioblastoma therapy. Hum Gene Ther, 23, 589-596. IF=5.7

16. Jiang, Y., Liu, J., Zhao, J., Zhang, W., Xia, H. (2010) Eukaryotic expression and identification of human Slit2 gene in HEK-293 cell line. Acta Universitatis Medicinalis Nanjing (Natural Science), 10.3724/SP.J.1238.2010.00512.

17. Liu, S., Mao, Q., Zhang, W., Zheng, X., Bian, Y., Wang, D., Li, H., Chai, L., Zhao, J., Xia, H. (2009) Genetically modified adenoviral vector with the protein transduction domain of Tat improves gene transfer to CAR-deficient cells. Biosci Rep, 29, 103-109. IF=2.942

18. Li, H., Zhang, W., Wang, D., Li, R., Qi, Z., Zhao, J., Xia, H. (2009) [Construction and expression of recombinant adenovirus vector carrying human endostatin, K5 and endostatin-K5 gene]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi, 25, 577-580.

19. Li, H., Zheng, X., Zhang, W., Bian, Y., Wang, D., Zhao, J., Xia, H. (2007) [Construction of an expression vector for high expression of siRNA]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi, 23, 1117-1118.

 

六、授权专利

1. 夏海滨,张伟锋,肖丹,单琳琳,一种抗凋亡细胞系及其建立方法和应用,2017.03.30-2037.03.29,中国,ZL 2017 10202547.3

2. 夏海滨,张伟锋,一种基因打靶系统,2015.6.10-2035.6.9,中国,ZL 2013 10157216.4

3. 夏海滨,张伟锋,锌指蛋白的快速筛选方法,2015.5.27-2035.5.26,中国,ZL 2012 1 0207952.1

4. 夏海滨,张伟锋,刘思也,携带锌指核酸酶表达元件和供体DNA的腺病毒及其构建方法和应用,2014.12.10-2034.12.9,中国,ZL 2012 1 0447965.6

5. 夏海滨,张伟锋,快速检测锌指核酸酶介导基因定点整合的方法,2013.6.12-2033.6.11,中国,ZL 2011 1 0009773.2

6. 夏海滨,张伟锋,王东阳,郑晓晶,赵俊丽,肿瘤特异嵌合启动子及其构建方法和应用,2011.11.30-2031.11.29,中国,ZL 2009 1 0023841.3

7. 夏海滨,张伟锋,郑晓晶,赵俊丽,王东阳,表达小干扰RNAVA1缺失的腺病毒载体及构建方法和应用,2011.5.4-2031.5.3,中国,ZL 2009 1 0022759.9